Bacterial Analysis for Food
An increasing appreciation for the importance of food and microbio- logical safety has resulted in a demand for a rapid, high-through- put method for total viable count (TVC) quantification to deal with
the increasing numbers of samples that require testing.
The industry standard for TVC determination
(ISO:4833:2033), also known as aerobic plate
count (APC), is widely used but presents users
with some very significant drawbacks. The
method is both material and labor intensive, requiring the preparation and analysis of multiple
agar plates per sample. More importantly, the
method is slow, with 48–72 hours typically required for a definitive result.
MOCON, in association with Luxcel
Biosciences, has addressed these limitations with
the development of the GreenLight™ 900 Series,
a breakthrough, low-cost system with reduced
labor and material, providing microbiological analysis in just hours.
Rapid determination of TVC/APC levels has an immediate impact on
your business in the following ways:
• Measures microbiology quality immediately after delivery
• Fast decision-making toward suppliers
• Standardizes internal microbiology quality control for goods received
• Immediate grading of incoming products
• Rapid hygiene indicator of products
• Contributes to competitive advantage
• Constant monitoring of production process (hygiene)
This process results in safer foods for consumers.
How Does It Work? (The Really Simple Explanation)
This sensor technology is based on oxygen sensing. As microbes grow,
they consume O2. The system measures bacterial O2 consumption and
equates that to viable microbial load (live bacteria).
A strong correlation is observed between the GreenLight and the
ISO:4833:2003 methods for the assessment of bacterial contamination, for
example, in ground beef samples; Pearsons correlation coefficient = 0.961.
Available in Two Models
• Model 960 (AOAC approved), a 96 micotiter plate-based assay that provides rapid high throughput.
• Model 910 (AOAC approval pending), a vial-based system providing low
to medium throughput.
Simple to Operate
The system does not require laboratory trained technicians to operate it.
Model 960 is a simple ‘mix and measure’ assay 96-well plate system.
Using food homogenates, prepared as for traditional TVC/APC testing (with
no further dilutions necessary) (ISO:4833:2033), probe, sample and oil are
added in sequence and resultant aerobic TVC/APC values quantified using
an appropriate calibration. The plate is
measured kinetically on a fluorescence
To obtain a copy of the GreenLight
White Paper or for more information on
the specific models, contact MOCON at
email@example.com or phone us at 763-493-
6370 or www.mocon.com/foodsafety.php.