insufficient temperature treatment will be diverted away from
filling. Another effective method for verifying the effectiveness of product sterilization is through media fill trials, where
a microbiologically sensitive medium, such as Linden Grain, is
sterilized, aseptically filled into sterile packs and plated for total
viable count, yeast and mold. The microbial content of the
media-filled packs must show an absence of growth.
Conditions required for the sterilization of filling machines
can be verified by placing Bacillus stearothermophilus spore strips
of known spore loads in target locations and measuring the log
reduction following equipment sterilization.
Alarms on filling machines with a hydrogen peroxide im-
mersion system can be tested to show they sound when the
hydrogen peroxide bath level is below a minimum volume.
A more quantitative method for verifying the effectiveness of
packaging sterilization is by inoculation pack testing, where preformed packaging is inoculated with a known amount of bacterial spores and introduced into the packaging machine where
it is sterilized. The packaging then undergoes microbiological
testing to determine the log reduction post-sterilization. Results
from package integrity testing where filled packs are inspected
for leakages or incorrect sealing can also verify that the product
has been hermetically sealed at the filler. Record keeping is an
important part of the verification process, as it serves as documented evidence that critical limits have been reviewed and
verified to be fully functional at maintaining aseptic control.
Table 2: Example of Product Sterilization Process Documentation
UHT: ultra-high temperature; PLC: programmable logic controller; RTD: resistance temperature detectors
of the RTD
exit of the
Diversion of the
product and stop
The Insights You Need to Succeed
In The Food Microbiology Testing Market
Now more than ever, accurate and in-depth information about food micro
testing methods, trends and technologies is critical to success. At Strategic
Consulting, we’ve been tracking this market for more than 15 years.
Our market research reports can help.
JUST PUBLISHED – NEW OVERVIEW
•;Food Micro, Seventh Edition:
Comparison of the Food Microbiology Testing Markets in the U.S. and E.U.
ALL NEW EDITIONS
•;Food Micro, Fifth Edition: Microbiology Testing in the U.S. Food Industry
To order or request a prospectus,
call (802) 457-9933 or visit us online at
Extensive Primary Research
We conduct detailed
interviews with food-
processing plants, senior
quality officers, key opinion
and contract test labs.
Detailed Data Summarized
in Clear Charts and Graphs
The Information You Need
to Make Sound Business
Track key drivers and trends,
and project future growth.
Strategic Consulting, Inc.;•;www.Strategic-Consult.com;•;(802) 457-9933