A sample from a client who regularly requests testing for confirmation that their product is gluten free received a result that indicated high levels of gluten contamination using the R5 enzyme-linked
immunosorbent assay (ELISA) method. The client was puzzled and
concerned because their in-house screening process was unable to
detect gluten on either this particular SKU or this lot.
Further analysis of all ingredients revealed that
rice syrup was the contaminated ingredient. The client wanted to know whether Bia Diagnostics could
determine the source of the contamination (rye,
barley or wheat), suspecting barley syrup cross-con-
tamination from a supplier as a possible culprit. Bia
was able to test the rice syrup alongside two differ-
ent ELISA methods, one using the R5 antibody and
the other using the Skerritt antibody (a monoclonal
antibody for gluten detection that can be used on a variety of cooked
and uncooked foods).
The results were telling: The Skerritt ELISA values for the sample
were less than 10 ppm, whereas the R5 ELISA values were greater
than 200 ppm.
Why did the client’s screening method not reveal contamination?
They used a screening method that utilized the Skerritt antibody
that underdetects barley gluten by about 20-fold, highlighting the
importance of utilizing the best fit-for-purpose method or combination of methods for your application.
The prevalence of allergic diseases in the industrialized world
has been on the rise for the last 50 years, according to the American
Academy of Allergy, Asthma and Immunology. The U.S. Centers for
Disease Control and Prevention state that food allergies are a growing food safety and public health concern that affect an estimated
4–6 percent of children in the United States. Coupled with the finalization of the Food Safety Modernization Act, monitoring of food
safety systems is more critical now than ever.
Bia Diagnostics is available to assist all organizations with their
Hazard Analysis and Critical Control Points plans for food allergen
screening and label verification.
The Expedited Expertise You Need
Bia Diagnostics is ISO 17025 accredited and AOAC, Good Laboratory Practices and Good Manufacturing Practices compliant. The
company’s food diagnostics facility, located in beautiful Vermont,
brings over 50 years of laboratory experience and nearly 30 years of food allergen
testing expertise. Using state-of-the-art
technologies in food analysis, Bia offers
the most reliable, highest-quality results
possible. By specializing in food allergens,
the company can offer rapid turnaround
times including same-day analysis. Clients
receive Certificates of Analysis for each
sample as well as complete data reduction.
Partnering with You to Find the Best
With so many ingredient, flavor and
preservative combinations possible, food
matrices can be very complex. Bia Diagnostics works closely with your team to
develop a customized plan to meet your
specific testing needs. This can include
evaluation and validation of the method
with each specific matrix that the assay
would encounter in your facility, thus
ensuring the most accurate results and
avoiding costly recalls.
At the Service of Our Customers and
the Scientific Community
Customer service is the keystone of
Bia Diagnostics’ corporate mission. To
that end, the company prides itself on
providing fast and reliable responses
to client needs, including unparalleled
service and the development of personal
relationships with all customers, regardless of size.
Bia Diagnostics strives to be a caring and active member of the scientific
community by conducting independent
and collaborative research to further the
understanding of food allergen safety
and of the community. By authoring scientific papers and presenting at scientific
conferences such as AOAC International,
International Food Technology Association and the American Association of
Cereal Chemists, Bia works each day to
develop innovative tools to help food
manufacturers produce the safest products for even the most allergen-sensitive
And now, Bia Diagnostics has added
real-time PCR testing for food authenticity and detection of genetically modified
organisms to the services offered.
Bia Diagnostics is your partner in
product quality control! It is your lab!
A Closer Look at Food Allergen
Screening: Gluten Contamination
Leaders in Food Allergen Analysis